Darren Way’s side welcome Phil Brown’s Sky Bet League One Shrimpers to Huish Park on Saturday.
It will be the first meeting between the two teams since a League Cup meeting in 2013 which ended 1-0 in the Glovers’ favour.
Yeovil have faced Southend four times in the competition, winning twice (1958 and 1963) (D1 L1).
Fans can purchase tickets online, by phone on 01935 84788 or in person at the ticket office.
For more information and prices, head to the Home Tickets section.