The Glovers turn their attention to the cup after extending their unbeaten run in Sky Bet League Two to eight matches on Saturday, with an emphatic 5-0 win against Crawley Town.
Now sitting in the play-offs, Town return to Checkatrade Trophy action after qualifying top of Southern Group A.
Victories over Portsmouth and Reading along with a point from a trip to Bristol Rovers was enough for Darren Way’s side to earn a home draw in Round Two.
The Dons travel to Somerset having finished second in their group, with a win, draw and loss to their name.
In League One MK sit towards the bottom of the table, but after the dismissal of long-term manager Karl Robinson, former Hearts manager Robbie Neilson will take charge of the Dons for just the second time on Tuesday night.
The Glovers have met MK Dons on 14 occasions dating back to 2006, with every previous meeting coming in League One.
Yeovil have come out on top on four occasions, with Town last winning against the Dons in January 2013. Paddy Madden scored both goals in a 2-1 win, with Antony Kay netting for the visitors.
MK won both of the previous meetings, following up a 2-0 win at Huish Park with a convincing 5-1 victory at Stadium MK on the last day of the season in 2015.
Lee Swabey will take charge of Tuesday night’s fixture at Huish Park.
The meeting will be just his second match of the season, having overseen the 2-2 draw between AFC Wimbledon and Fleetwood Town at the end of November.
Mr Swabey’s last game involving the Glovers came in March last season, officiating the 3-0 loss away to Cambridge United which also saw Liam Walsh sent off.
On Tuesday Mr Swabey will be assisted by Andrew Laver (Hampshire) and Colin Lymer (Hampshire), with Brett Huxtable acting as fourth official.
On matchday, minute-by-minute updates will be provided on YTFC’s official Twitter account which can be found here, with additional updates available via YTFC’s official Facebook page.
If you’re unable to attend the game a full match report will follow on ytfc.net at full-time with post-match reaction from manager Darren Way and a member of the playing staff following soon after.
Supporters wishing to attend the match can purchase tickets via the Huish Park ticket office, online or by calling 01935 847888.