Darren Way’s men finished top of the group before seeing off Sky Bet League One outfit AFC Wimbledon in the second round.
They go into their meeting with Forest Green Rovers in high spirits following their Emirates FA Cup victory over third tier outfit Bradford City.
Southampton loanee Marcus Barnes netted on his Yeovil debut but will miss out due to being cup-tied, along with new arrival Lewis Wing.
Fellow new recruits Jared Bird and Corey Whelan are available.
Defender Omar Sowunmi and Connor Smith are available after missing the win against Bradford due to suspension.
Striker Francois Zoko could make his 100th appearance for the club.
This term is Forest Green’s first competing in the EFL Trophy.
Mark Cooper’s Green Devils finished second in their group before defeating his former employer Swindon Town to set up tonight’s tie at Huish Park.
Christian Doidge is subject to a lot of interest from Championship clubs, while midfielder Dayle Grubb will also provide a threat after a sterling 2017.
FGR won the first EFL meeting between the sides back in August at the New Lawn.
Fourteen years previous, Town were triumphant at Huish Park on New Year’s Day.
The Glovers ran out 1-0 winners courtesy of a solitary effort from Kirk Jackson.
John Busby will be the man in the middle.
The Oxfordshire-based official has taken charge of 29 games so far this season, awarding 74 yellow cards and four reds.
Busby hasn’t refereed Town this season but officiated three games last term; the 1-0 home defeat to Morecambe, the 2-0 away win at Reading Under-21s and the 2-0 defeat at Crawley Town.
He will be assisted by Andrew Laver (Hampshire) and Justin Amey (Dorset) with Lee Swabey (Devon) acting as fourth official.
On matchday, minute-by-minute updates will be provided on YTFC’s official Twitter account and live matchday centre.
If you’re unable to attend the game, live audio commentary is available via the iFollow Glovers service, with the monthly and season subscriptions offering full-match replays from Sunday.
A full match report will follow on YTFC.net at full time with post-match reaction from the manager and a member of the playing staff following soon after.
Fans can purchase from the ticket office this evening.