A Marc Laird goal after 87 minutes was enough to earn the Glovers the win against their Cornish hosts.
Manager Paul Sturrock named a first half XI and a different second half XI with only a couple of players playing all 90 minutes.
Included in the starting line up were trialists Paul Connolly, Iffy Allen and Ashley Vincent.
The game started at a frantic pace with both teams passing the ball around nicely in the opening exchanges.
The first real chance of the match fell to striker Shaun Jeffers after 15 minutes. His turn and shot from the edge of the box went narrowly wide of the Truro post.
Just before the half time interval the home side when Shane White headed home from close range. But the referee’s assistant had other ideas as he raised his flag for offside.
At half time Town changed their team around with new trialists Wilson Carvalho, Christopher Ibaiya getting a chance to try and impress the Yeovil Town management team.
Fifteen minutes into the second period Jakub Sokolik thought he had opened the scoring when his cross/shot cannoned off the cross bar and away to safety.
Five minutes later Jamie Burrows was close to opening his account for the Glovers, but his powerful header was well saved by Tom McHale in the Truro goal.
With just three minutes to go and the score line still at 0-0 Town scored the only goal of the game.
A cross from the left wing from Carvalho was met by the leaping Burrows, who headed back across goal into the path on Laird, who made no mistake from close range.
Manager Paul Sturrock said: “I am happy everyone got 45 minutes under their belt.
“We haven’t really worked on any shape as of yet, so it was really pleasing to get of to a winning start.
“We are back training tomorrow (Saturday) and have another game at Callington on Sunday, before some testing home pre-season games.
“I thought Paul Connolly played well. He hasn’t trained with a team for a while. I thought he didn’t put a foot wrong.”