The head coach has selected an 18-man group by head coach Cooper for the game at Huish Park in Yeovil.
And England will want to end the competition with a win which could see them lift the Shield depending on the outcome of the game between Northern Ireland and Wales.
That will not be Cooper’s main priority though, as the latest batch of English youngsters take their early steps on the international ladder.
A number of the squad have already featured in the first two games in the Shield, a 1-0 defeat to Wales in Bangor and then a win over Northern Ireland earlier this month.
However, there are first call-ups for Manchester City duo Diego Lattie and Ayotomiwa Bashiru and Arsenal forward Reiss Nelson.
"The players were really looking forward to meeting up for this game and relationships have already started to form which is great," said Cooper.
England U16s head coach Steve Cooper.
Steve Cooper has selected an 18-man squad for the game in Yeovil
"Development is about individual talent but it is also about playing in a team. To do that we need to make sure the players bond – and they certainly are.
"We are working with 16-year-olds who are on the start of an international journey and we need to be mindful of that.
"Our expectations need to be high but they need to be appropriate. We need to concentrate on the now but have an eye on the future as well."
Goalkeepers: Taye Ashby-Hammond (Fulham), Ryan Sandford (MIllwall).
Defenders: Tolaji Bola (Arsenal), Trevoh Chalobah (Chelsea), Diego Lattie (Manchester City), Vashon Neufville (West Ham United), Japhet Tanganga (Tottenham Hotspur).
Midfielders: Ayotomiwa Bashiru (Manchester City), Andre Dozzell (Ipswich Town), Charlie Gilmour (Arsenal), Mason Mount (Chelsea), Callum Slattery (Southampton), Joe Willock (Arsenal).
Forwards: Martell Taylor Crossdale (Chelsea), Jonathan Leko (West Bromwich Albion), Adam Lewis (Liverpool), Reiss Nelson (Arsenal), Dan N’Lundulu (Southampton).
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