The Glovers have already met the Valiants once this season, playing out a 1-1 draw at Huish Park with Otis Khan cancelling out Tom Pope’s opener.
However, the Green and Whites will come into the clash at Vale Park in buoyant mood having seen off Sky Bet League One outfit Plymouth Argyle 2-1 in the Checkatrade Trophy on Tuesday.
In fact, Darren Way’s side progressed to the second round of the FA Cup in similar circumstances, defeating higher league opposition in Southend United at the start of November.
Khan’s first-half penalty was enough to knock out Phil Brown’s Shrimpers, teeing up a second round meeting in Stoke-on-Trent.
With no fresh injury concerns, Yeovil will travel to Port Vale with only Sid Nelson absent from the matchday squad as he serves the second of a three-match suspension.
However, Way will have something of a selection headache having made five changes for the midweek win over Argyle, while new signing Oscar Gobern is eligible to make his debut having signed on Friday morning.
Port Vale host the Glovers following back-to-back defeats.
The Valiants will look to continue their FA Cup form, though, having recorded a 2-0 win over Oxford United in the first round.
Pope (9 goals) will be the hosts’ main threat, with Dan Turner also chipping in with three goals this season.
Town have won three of their nine encounters against Port Vale.
The first meeting between the two sides came as early as the 2005/06 season with both sides winning their respective home fixtures 1-0.
Yeovil’s best result at Vale Park is a 2-2 draw, coming back in 2008 thanks to Gary Dempsey and Andy Kirk strikes.
Scott Oldham (Lancashire) will be the man in the middle on Saturday.
The cup clash will be his 19th game of the season and first involving the Glovers.
Mr Oldham will be assisted by Richard Wigglesworth (South Yorkshire) and Andrew Hendley (West Midlands) with Peter Wright (Merseyside) acting as fourth official.
Darren Way and Olufela Olomola’s pre-match thoughts can be viewed on iFollow Glovers.
If you’re unable to attend the game, live audio commentary is available via the iFollow Glovers service, with the monthly and season subscriptions offering full-match replays from Sunday.
A full match report will follow on YTFC.net at full time with post-match reaction from the manager and a member of the playing staff following soon after.
Tickets for the match are available to buy in advance and on the day, with details on our away ticket page.