After three consecutive draws in the league, Town will be looking to get back to winning ways at Whaddon Road.
The Glovers have had to settle for a point in their last two encounters at home after conceding late goals on both occasions.
Darren Way and his squad have notified the issue as a lack of concentration and focus going into the last ten minutes of matches, and want to address it against Cheltenham.
Former Yeovil boss Gary Johnson’s first return to the club since leaving in February 2015 ended in defeat back in September.
The 61-year-old guide the Green and Whites to three promotions - including the historic one to the Championship - and one FA Trophy across two spells with the club.
Johnson’s Robins won the Vanarama National League title last season but are fighting against a return to non-league.
They lie one place above the relegation zone and have lost six of their last 10 fixtures.
Last time out, the Gloucestershire club fell to a 2-1 away defeat at Stevenage.
The two sides hadn’t previously played each other for seven years before this season, with Town in the Sky Bet Championship and League One and Cheltenham suffering relegation prior to the Glovers returning to the bottom division for the 2015/16 campaign.
Yeovil have won four of the 11 competitive meetings between the two sides, the last coming back in September.
Otis Khan opened the scoring for Town after six minutes and Darren Ward’s powerful header doubled their advantage.
Aaron Downes pulled one back for the Robins but a diving header from Matty Dolan made it 3-1 on the stroke of half-time.
Cheltenham reduced the deficit once more through Billy Waters before Tom Eaves rounded off the scoring in the last minute.
Darren England (South Yorkshire) will take charge of Saturday’s meeting at Whaddon Road, officiating his 31st match of the campaign.
He has awarded 76 yellow cards and five reds, and is yet to take charge of a Town game this season.
The Yorkshire-born official will be assisted by Ian Smedley (Derbyshire) and John Law (Worcestershire) with Andrew Newbold (Leicestershire taking on the role of fourth official.
Darren Way and Ben Whitfield’s pre-match interviews can be viewed on YTFC's official YouTube account here.
On matchday, minute-by-minute updates will be provided on YTFC's official Twitter account which can be found here, with additional updates available via YTFC's official Facebook page.
If you’re unable to attend the game a full match report will follow on ytfc.net at full-time with post-match reaction from manager Darren Way and a member of the playing staff following soon after.
Ticket information can be found by clicking here. Supporters wishing to attend the match can purchase tickets online by clicking here, or via the Huish Park ticket office either in person or by calling 01935 847888.